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active akt ca akt plasmid  (Addgene inc)


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    Addgene inc active akt ca akt plasmid
    Active Akt Ca Akt Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+akt+plasmid/pCDH-puro-myr-HA-Akt1+(Plasmid+%2346969)/pm41061292-170-9-16
    Average 92 stars, based on 18 article reviews
    active akt ca akt plasmid - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Anticancer effect of involucrasin A on colorectal cancer cells by modulating the Akt/MDM2/p53 pathway.
    Article Snippet: .. The CA‐Akt plasmid was obtained from Addgene, Inc. (cat. no. 46969). ..

    Article Title: Dihydroartemisinin inhibits the migration of esophageal cancer cells by inducing autophagy
    Article Snippet: The grey value was detected using ImageJ software v1.51d (National Institutes of Health). .. A total of 9×10 5 cells were seeded in 6-well plates overnight, and 5 μg CA-Akt plasmid (cat. no. 14751; Addgene, Inc.) and pcDNA3.1 empty vector (cat. no. V790-20, Invitrogen; Thermo Fisher Scientific, Inc.) was transfected into the cells using Lipofectamine ® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) when the cell fusion rate reached 85%. ..

    Article Title: Anticancer effect of involucrasin A on colorectal cancer cells by modulating the Akt/MDM2/p53 pathway
    Article Snippet: .. The CA-Akt plasmid was obtained from Addgene, Inc. (cat. no. 46969). ..

    Transfection:

    Article Title: Dihydroartemisinin inhibits the migration of esophageal cancer cells by inducing autophagy
    Article Snippet: The grey value was detected using ImageJ software v1.51d (National Institutes of Health). .. A total of 9×10 5 cells were seeded in 6-well plates overnight, and 5 μg CA-Akt plasmid (cat. no. 14751; Addgene, Inc.) and pcDNA3.1 empty vector (cat. no. V790-20, Invitrogen; Thermo Fisher Scientific, Inc.) was transfected into the cells using Lipofectamine ® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) when the cell fusion rate reached 85%. ..

    Cloning:

    Article Title: MAPK4 deletion enhances radiation effects and triggers synergistic lethality with simultaneous PARP1 inhibition in cervical cancer
    Article Snippet: The coding sequence (CDS) of MAPK4 was amplified using a human genomic DNA extracted from SiHa cells and then inserted into the PCI plasmid (Promega, Madison, WI, USA). .. Primers sequences for cloning MAPK4 CDS into the expressing vector are listed in Table S . CA-AKT plasmid was purchased from Addgene (catalog no. plasmid #14751, Watertown, MA). .. After cells were incubated with different concentrations of PARP1 inhibitors, olaparib or veliparib, cell viablity was determined by a cell counting kit-8 (CCK-8, Dojindo, Tokyo, Japan).

    Expressing:

    Article Title: MAPK4 deletion enhances radiation effects and triggers synergistic lethality with simultaneous PARP1 inhibition in cervical cancer
    Article Snippet: The coding sequence (CDS) of MAPK4 was amplified using a human genomic DNA extracted from SiHa cells and then inserted into the PCI plasmid (Promega, Madison, WI, USA). .. Primers sequences for cloning MAPK4 CDS into the expressing vector are listed in Table S . CA-AKT plasmid was purchased from Addgene (catalog no. plasmid #14751, Watertown, MA). .. After cells were incubated with different concentrations of PARP1 inhibitors, olaparib or veliparib, cell viablity was determined by a cell counting kit-8 (CCK-8, Dojindo, Tokyo, Japan).



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    Image Search Results


    Constitutively active AKT (CA-AKT) significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μ M ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3 β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μ M ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

    Journal: Journal of Oncology

    Article Title: Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3 β /Tyr216 Accumulation

    doi: 10.1155/2021/4475192

    Figure Lengend Snippet: Constitutively active AKT (CA-AKT) significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μ M ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3 β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μ M ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

    Article Snippet: Endotoxin-free CA-AKT plasmids (used for transfection) were extracted using Endo-Free Plasmid Midi Kit-fast (Omega Bio-tek, Norcross, GA, USA).

    Techniques: Migration, Transfection, Plasmid Preparation, Western Blot, Expressing